108(3)_str 34

 

ISSN 1392-3196 / e-ISSN 2335-8947
Zemdirbyste-Agriculture, vol. 108, No. 3 (2021), p. 263–270
DOI 10.13080/z-a.2021.108.034

Fine mapping and candidate analysis of Osgl: a gene regulating grain shape and quality in japonica rice

Mustapha SUNUSI, Zhongkang WANG, Yohannes TSAGO, Jamal Adam ESHAQ, Jialin LIU, Asadullah KHAN, Ziyan CHEN, Chunhai SHI, Xiaoli JIN

 Abstract

Grain size is one of the important agronomic traits that determines japonica rice (Oryza sativa subsp. japonica) yield. Map-based cloning and genetic analysis of grain size-related genes have an impact on elucidating the genetic regulation mechanism of rice yield formation. In this study, the Osgl gene was identified by map-based cloning. The mutant has significant increase in grain length, grain width and grain length and width ratio, yield per plot, panicle length, number of grains per panicle and seed setting rate compared to the wild type (WT). There was no noticeable difference in grain thickness and 1000-grain weight between the Osgl mutant and wild type. Taken together, the result signifies that the Osgl mutant regulates organ size grain length possibly due to cell proliferation. Starch granules in the Osgl mutant are closely arranged and regular polyhedron-like, while the composite starch particles contained in the white, opaque parts of the wild-type endosperm are loosely arranged, and the shape changes to spherical or ellipsoid. Genetic analysis showed that the Osgl mutant was controlled by a recessive single gene and was finely located in chromosome 3 through map-based cloning within a physical distance of 40 kB. Sequencing analysis revealed that A to G substitution in the 4th exon of Os03g0407400 gene resulted in an amino acid change glycine to argenine in PEBP-like domain. Taken together, Osgl mutant has a pleiotropic effect on grain yield and grain quality.

Key words: gene mapping, grain shape, grain quality, Oryza sativa subsp. japonica.

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