ISSN 1392-3196 / e-ISSN 2335-8947
Zemdirbyste-Agriculture, vol. 106, No. 4 (2019), p. 359–366
Application of marker-assisted selection for resistance to gall mite and Blackcurrant reversion virus in Ribes genus
Ingrida MAZEIKIENE, Ana Dovile JUSKYTE, Vidmantas STANYS
Gall mite (Cecidophyopsis ribis Westw.) is a biological vector of Blackcurrant reversion virus (BRV), and both are widespread in the countries, where blackcurrants are cultivated commercially. Blackcurrant (Ribes nigrum L.) is the primary natural host of BRV, although natural infestations also occur in other Ribes spp. Some Ribes species and cultivars are resistant to C. ribis and/or BRV. The mechanism and gene structure of resistance are not clear, that is why molecular markers for breeding of resistant blackcurrants are still in the process of searching. Several molecular markers related to resistance to the pathogen and pest in Ribes spp. genotypes were identified by quantitative trait loci (QTL) mapping. These molecular markers can be used for acceleration of the breeding process of resistant blackcurrant, because phenotyping in the field is unnecessary. In our study, the presence of the 12 polymerase chain reaction (PCR) markers, amplified fragment length polymorphism (AFLP) and single sequence repeat (SSR), linked to gall mite and virus resistance loci from 4 to 20 cM were analysed. Eleven of them demonstrated suitability for selection of resistant genotypes in this study. The presence of these molecular markers in 8 Ribes species, 15 hybrids and 13 R. nigrum cultivars was shown. Fully specific molecular marker for detection of genotypes with resistance to gall mite and virus among Ribes species was not found. The complex application of eleven molecular markers allowed for significant grouping of Ribes species, cultivars and hybrids with different genetic origin of resistance to pest and virus. As well, they can be useful for marker-assisted selection. Six molecular markers: E36M59_107, E40M43_236, E40M43_289, E40M40_219, E41M88_280 and E45M40_222, were distinguished as more suitable for identifying resistant Ribes spp. genotypes with Ce and P genes in this study.
Key words: gall mite, molecular marker, resistance.
Full text: 106_4_str46.pdf