106(3)_str34

ISSN 1392-3196 / e-ISSN 2335-8947
Zemdirbyste-Agriculture, vol. 106, No. 3 (2019), p. 265–272
DOI 10.13080/z-a.2019.106.034

Expression profiling of two sucrose transporter genes during post-anthesis in wheat

Saeid NAVABPOUR, Parinaz JALALI

Abstract

Sucrose transporters (SUTs) are critical for distribution and storage of sucrose between the source and sink tissues and considered as an important key for an efficient growth of common wheat (Triticum aestivum L.) during post-anthesis as well as during senescence stage. Therefore, a complete study including molecular and biochemical experiments was carried out to assess the TaSUT1 and TaSUT2 genes expression, reactive oxygen species: superoxide radical (O₂•^‒) and hydrogen peroxide (H₂O₂), production and antioxidant enzymes: superoxide dismutase (SOD) and catalase (CAT), activity as well as non-enzymatic antioxidants: ascorbate, α-tocopherol and carotenoid, content in flag leaf and other leaves of promising line N81-18 of wheat during post-anthesis. The TaSUT1 and TaSUT2 genes expression was identified in both flag leaf and other leaves, as the flag leaf had the highest genes expression. The activity of SOD and CAT significantly increased in both flag leaf and other leaves, which caused a decrement of O₂•^‒ and H₂O₂ content, respectively. Moreover, the ascorbate, α-tocopherol and carotenoid content significantly increased as a cellular defence mechanism to minimize the damage due to oxidative stress caused during post-anthesis and leaf senescence. Overall, it can be concluded that flag leaf had the highest TaSUT1 and TaSUT2 genes expression as well as non-enzymatic antioxidants that could be considered as the main key for future genetic studies and practical breeding programs.

Key words: antioxidant, ascorbate content, α-tocopherol, catalase, hydrogen peroxide, superoxide radical.

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