104(3)_str31

 

ISSN 1392-3196 / e-ISSN 2335-8947
Zemdirbyste-Agriculture, vol. 104, No. 3 (2017), p. 243–248
DOI  10.13080/z-a.2017.104.031

Genotypic and exogenous factors affecting linseed ovary culture

Aušra BLINSTRUBIENĖ, Natalija BURBULIS, Ramunė MASIENĖ

Abstract

The current study investigated the effect of genotype, growth regulators and type of carbohydrates on callus induction and indirect shoot regeneration in ovary culture of 8 linseed (Linum usitatissimum L.) cultivars. Callogenic response varied from 9.17% to 100% depending on the cultivars and medium composition interaction. The replacement of sucrose with a combination of sucrose + maltose significantly improved the callogenesis in 3 or 4 investigated cultivars, depending on growth regulators in the induction medium. The frequency of shoot formation from ovary-derived callus in 5 responsive cultivars ranged from 4.17% to 75.00%, whereas the other three cultivars tested did not exhibit any shoots. The replacement of sucrose with a sucrose + maltose combination in induction medium reduced or completely inhibited shoot formation frequency of responsive cultivars. The significantly highest mean shoot formation frequency (52.50%) was obtained from ovary-derived callus of the cultivar ʻMikael’. The analysis of variance revealed that cultivar (C), combination of growth regulators (GR), type of carbohydrates (CH) and their interaction significantly influenced callus induction and shoot formation frequency. In most cases, a higher shoot regeneration frequency was obtained when callus was from induction medium supplemented with 2 mg l-1 thidiazuron (TDZ) + 1 mg l-1 α-naphthylacetic acid (NAA) with 6% sucrose. Cytological analysis of root tips showed that 21.88% of the regenerated plants were haploids, while another group of regenerants (78.12%) were diploid and mixoploid.

Key words: carbohydrates, genotype, growth regulators, Linum usitatissimum, morphogenic response, ovary culture.

Full text: 104_3_str31.pdf